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The actin cytoskeleton is one of the primary constituents of plant cells, and it has a central role in cell shape and movements. Actin filaments are often not visible or of low abundance in electron micrographs of plant cells. Here we describe a technique for identifying actin filaments in pollen tubes of Lolium multiflorum Lam. and Lemna minor L. The technique is based on the binding of wheat germ agglutinin (WGA) to actin filaments. The WGA method has been applied to the pistil tissue of many plants, but has not been used in combination with pollen tubes. 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